Alexa Fluor® 647 anti-mouse Ly-6C Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse Ly-6C Antibody
- 产品类别：
- 抗体
- 产品编号：
- 128009
- 产品应用：
- 128009
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
- Turner JA, et al. 2020. Immunity. 53:1202. PubMed
- Klopfenstein N, et al. 2021. PLoS Pathog. 17:e1009387. PubMed
- Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
- Lissner MM, et al. 2020. Elife. 9:00. PubMed
- Nguyen GT, et al. 2021. iScience. 24(8):102871. PubMed
- Stutz MD, et al. 2021. Immunity. 54(8):1758-1771.e7. PubMed
- Indramohan M, et al. 2012. Infect Immun . 80:4099. PubMed
- Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
- Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
- Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
- Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
- Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
- Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
- Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
- Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
- RRID
- AB_1236551 (BioLegend Cat. No. 128009) AB_1236550 (BioLegend Cat. No. 128010)
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
- Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
