Purified anti-human CD8a Antibody
- 产品名称：
- Purified anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300901
- 产品应用：
- 300901
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Ibidapo-Obe O, et al. 2020. Cell Mol Gastroenterol Hepatol. 9:661. PubMed
- Gao H, et al. 2021. J Virol. :. PubMed
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Song S, et al. 2021. Commun Biol. 4:1338. PubMed
- Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
- Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
- Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
- Freund L, et al. 2020. J Invest Dermatol. 1455:140. PubMed
- Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
- Chiou SH, et al. 2021. Immunity. 54:586. PubMed
- RRID
- AB_314105 (BioLegend Cat. No. 300901) AB_314106 (BioLegend Cat. No. 300902)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
