PerCP/Cyanine5.5 anti-human CD8a Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300923
- 产品应用：
- 300923
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
- Balakrishnan A, et al. 2017. Biol Blood Marrow Transplant. 10.1016/j.bbmt.2017.07.016. PubMed
- Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
- Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
- Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
- Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
- Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
- Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
- Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
- Imura Y, et al. 2020. JCI Insight. :5. PubMed
- Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
- Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
- Keskin DB, et al. 2019. Nature. 565:234. PubMed
- RRID
- AB_1575079 (BioLegend Cat. No. 300923) AB_1575074 (BioLegend Cat. No. 300924)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
