PerCP anti-human CD8a Antibody
- 产品名称：
- PerCP anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300921
- 产品应用：
- 300921
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1836 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Holder KA, et al. 2021. Clin Transl Immunology. 10:e1348. PubMed
- Wang K, et al. 2020. Signal Transduct Target Ther. 0.404861111. PubMed
- Kashima Y, et al. 2022. Life Sci Alliance. 5:. PubMed
- Sperk M, et al. 2021. iScience. 24:102111. PubMed
- Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
- Li P, et al. 2022. Front Oncol. 12:917400. PubMed
- Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
- Chen Y, et al. 2021. Front Mol Biosci. 8:777370. PubMed
- Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
- Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
- RRID
- AB_1575076 (BioLegend Cat. No. 300921) AB_1575072 (BioLegend Cat. No. 300922)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
