PE/Cyanine5 anti-human CD8a Antibody
- 产品名称：
- PE/Cyanine5 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300909
- 产品应用：
- 300909
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1512 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
- Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
- Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
- Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
- Teni Nurlatifah HR, et al. 2021. Med Arch. 75:335. PubMed
- Hr TN, et al. 2021. Allergol Immunopathol (Madr). 49:193. PubMed
- Wheway J, et al. 2013. PLoS One. 8:e52586. PubMed
- Wheway J, et al. 2014. J Immunol. 193:3378. PubMed
- Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
- RRID
- AB_314113 (BioLegend Cat. No. 300909) AB_314114 (BioLegend Cat. No. 300910)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
