PE anti-human CD8a Antibody
- 产品名称：
- PE anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300907
- 产品应用：
- 300907
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
- Tatari N, et al. 2022. Front Immunol. 13:905768. PubMed
- Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
- Zhu W, et al. 2021. Onco Targets Ther. 14:2149. PubMed
- Ali A, et al. 2020. J Infect Dis. 222:853. PubMed
- Dushyanthen S, et al. 2017. Nat Commun. 10.1038/s41467-017-00728-9. PubMed
- Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
- Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
- Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
- Dockry E, et al. 2018. Oncoimmunology. 7:e1428156. PubMed
- Dziedzic K, et al. 2021. International Immunopharmacology. 96:107645. PubMed
- Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
- Sun Y, et al. 2020. Cell. 184(2):404-421.e16. PubMed
- Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
- Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed
- Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
- Potashnikova DM, et al. 2022. Front Med (Lausanne). 9:891916. PubMed
- Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
- Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
- Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed
- RRID
- AB_314111 (BioLegend Cat. No. 300907) AB_314112 (BioLegend Cat. No. 300908)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
