FITC anti-human CD8a Antibody
- 产品名称：
- FITC anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300905
- 产品应用：
- 300905
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Zhang H, et al. 2021. Front Immunol. 12:644520. PubMed
- Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
- Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
- Galindo-Albarrán A, et al. 2016. Cell Rep. 17:2151-2160. PubMed
- Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
- Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
- Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
- Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
- Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
- Bratke K, et al. 2017. Clin Exp Allergy. . 10.1111/cea.13017. PubMed
- Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
- Durning S, et al. 2016. J Immunol. 197: 3076 - 3085. PubMed
- Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed
- Martin SD, et al. 2018. Oncoimmunology. 7:e1371895. PubMed
- Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
- Todnem Sakkestad S, et al. 2019. Pathogens. 8. PubMed
- Hu JQ, et al. 2020. J Cancer. 3.409722222. PubMed
- Pan X, et al. 2021. Front Pharmacol. 11:539261. PubMed
- RRID
- AB_314109 (BioLegend Cat. No. 300905) AB_314110 (BioLegend Cat. No. 300906)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
