APC anti-human CD8a Antibody
- 产品名称：
- APC anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300911
- 产品应用：
- 300911
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Li C, et al. 2022. J Exp Med. 219:. PubMed
- Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
- Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
- Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
- Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
- Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
- Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
- Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
- Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
- Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
- Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
- Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
- Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
- Uniken Venema WT, et al. 2019. Gastroenterology. 156:812. PubMed
- Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
- Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
- Lamsfus Calle C, et al. 2021. Front Immunol. 12:625712. PubMed
- Ernst W, et al. 2013. Infect Immun . 81:1520. PubMed
- Niu M, et al. 2021. Cell Reports. 36(8):109611. PubMed
- Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
- RRID
- AB_314115 (BioLegend Cat. No. 300911) AB_314116 (BioLegend Cat. No. 300912)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
