Alexa Fluor® 700 anti-human CD8a Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300919
- 产品应用：
- 300919
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
- Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
- Product Citations
-
- Kim MY, et al. 2021. JCI Insight. 6:. PubMed
- Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
- Trzupek D, et al. 2022. Wellcome Open Res. 6:149. PubMed
- Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
- Kinjyo I, et al. 2010. J Immunol. 185:7151. PubMed
- Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
- Du L, et al. 2021. Front Mol Biosci. 8:675179. PubMed
- Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
- de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
- Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
- Geng J, et al. 2018. Elife. 7:e36341. PubMed
- Meng Q, et al. 2016. Arch Virol. 161: 181-187. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
- Westera L, et al. 2017. Clin Transl Gastroenterol. 8:e126. PubMed
- Walk J, et al. 2019. Nat Commun. 10:874. PubMed
- Teirlinck A, et al. 2011. PLoS One. 7:e1002389. PubMed
- Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
- Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
- Wilmes S, et al. 2021. Elife. 10:. PubMed
- Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
- Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
- Wells DK, et al. 2020. Cell. 183:818. PubMed
- Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
- Li L, et al. 2022. JCI Insight. 7:. PubMed
- Gorby C, et al. 2020. Sci Signal. :13. PubMed
- Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
- Tosch C, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0274-x. PubMed
- RRID
- AB_528884 (BioLegend Cat. No. 300919) AB_528885 (BioLegend Cat. No. 300920)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
