- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
- Rivollier A, et al. 2004. Blood 104:4029. (Activ)
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
- Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
- Edgar CE, et al. 2015. PLoS One. 10:117614. PubMed
- Product Citations
-
- Parsons M, et al. 2010. J Leukoc Biol. 88:905. PubMed
- Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
- Carbone ML, et al. 2022. Sci Rep. 12:5448. PubMed
- Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
- Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
- Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
- Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
- Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
- Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
- Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
- Grier A, et al. 2016. Mol Ther Nucleic Acids. 5: e306. PubMed
- Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
- Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
- Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
- R?lle A, et al. 2018. Cell Rep. 24:1967. PubMed
- Subedi N, et al. 2021. Sci Rep. 11:17084. PubMed
- Ciaglia E, et al. 2021. J Gerontol A Biol Sci Med Sci. 76:1775. PubMed
- Walk J, et al. 2019. Nat Commun. 10:874. PubMed
- Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
- Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
- Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
- Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
- Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
- Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
- Paulikat AD, et al. 2022. J Innate Immun. . PubMed
- Edgar C, et al. 2015. PLoS One. 10:117614. PubMed
- Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed
- Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
- Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
- Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
- Xian S, et al. 2021. EMBO Rep. 22:e52509. PubMed
- Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
- Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
- Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
- Li M, et al. 2020. Nat Commun. 4051:11. PubMed
- Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
- RRID
- AB_1575010 (BioLegend Cat. No. 300327) AB_1575008 (BioLegend Cat. No. 300328)
