Biotin anti-human CD3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 996	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
Rivollier A, et al. 2004. Blood 104:4029. (Activ)
Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)

Product Citations

Rohde D, et al. 2022. Nat Cardiovasc Res. 1:28. PubMed
Agarwal S, et al. 2020. Cell Reports. 30(5):1292-1299.e3.. PubMed
Zebrowska A, et al. 2022. Cells. 11:. PubMed
Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
Mondal SK, et al. 2021. Methods Mol Biol. 2265:305. PubMed

RRID

AB_314039 (BioLegend Cat. No. 300303) AB_314040 (BioLegend Cat. No. 300304)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: T cells, NKT cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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