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APC/Cyanine7 anti-human CD3 Antibody
产品名称：
APC/Cyanine7 anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300317
产品应用：
300317
[价格]
规格 价格 库存
25tests ￥ 1836 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
  8. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  9. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
  10. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  11. Liu Y, et al. 2015. J Immunol. 194:5851. PubMed
Product Citations
  1. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  2. Aoki T, et al. 2020. Cancer Sci. 111:2223. PubMed
  3. Sun A, et al. 2021. J Dent Sci. 16:751. PubMed
  4. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  5. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  6. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  7. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  8. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  9. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  10. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  11. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  12. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  13. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  14. Liu R, et al. 2021. Commun Biol. 4:652. PubMed
  15. Hinderer S, et al. 2012. Biomaterials. 33:5259. PubMed
  16. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  17. Kreutmair S, et al. 2021. Immunity. . PubMed
  18. Phad GE, et al. 2022. Nat Immunol. 23:1. PubMed
  19. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  20. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  21. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  22. Shen J, et al. 2021. Sci Adv. 7:eabi9787. PubMed
  23. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  24. Stunnenberg M, et al. 2021. Eur J Immunol. 51:2464. PubMed
  25. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  26. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  27. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  28. Pache L, et al. 2020. Cell Rep Med. 1:100037. PubMed
  29. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  30. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  31. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  32. Bradley S, et al. 2015. PLoS One. 10: 0141171. PubMed
  33. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  34. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  35. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  36. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  37. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  38. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  39. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  40. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  41. Liu Y, et al. 2015. J Immunol. 194:5851. PubMed
  42. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
  43. Singh KS, et al. 2021. Nature. 589:597. PubMed
  44. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  45. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  46. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  47. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  48. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  49. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  50. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  51. Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
  52. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  53. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
RRID
AB_314053 (BioLegend Cat. No. 300317) AB_314054 (BioLegend Cat. No. 300318)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution
Mature T and NK-T cells, thymocyte differentiation
Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
T cells, NKT cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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