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APC anti-human CD3 Antibody
产品名称：
APC anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300311
产品应用：
300311
[价格]
规格 价格 库存
25tests ￥ 396 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Pan J, et al. 2021. J Cell Mol Med. 25:1089. PubMed
  2. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  3. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  4. Lopez-Vergès S, et al. 2010. Blood. 116:3865. PubMed
  5. Yomogida K, et al. 2012. Cytokine. 58:431. PubMed
  6. Merugu S, et al. 2020. Methods Enzymol. 639:289. PubMed
  7. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  8. Ge Y, et al. 2019. J Immunol. 203:2827. PubMed
  9. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  10. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  11. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  12. Cao Y, et al. 2021. Cell Res. 31:732. PubMed
  13. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  14. Zhang J, et al. 2022. Nature. 609:369. PubMed
  15. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  16. Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed
  17. Morton JJ, et al. 2020. Molecular Cancer Research. 19(2):346-357. PubMed
  18. Roider T, et al. 2021. Blood Adv. 5:5060. PubMed
  19. Wang L, et al. 2021. Microbiol Spectr. 9:e0124621. PubMed
  20. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  21. Mlynska A, et al. 2020. Am J Reprod Immunol. 84:e13244. PubMed
  22. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  23. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  24. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  25. Hu Q, et al. 2021. Nat Commun. 12:2186. PubMed
  26. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  27. Lee YG, et al. 2019. Nat Commun. 10:2681. PubMed
  28. Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
  29. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  30. Hu JF, et al. 2022. Front Immunol. 13:958960. PubMed
  31. Li X, et al. 2021. Front Oncol. 11:703087. PubMed
  32. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  33. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  34. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  35. Jiang L, et al. 2019. Sci Rep. 9:3705. PubMed
  36. Wang Z, et al. 2018. Front Immunol. 9:1239. PubMed
  37. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  38. Mlynska A, et al. 2019. Oncol Rep. 41:1238. PubMed
RRID
AB_314047 (BioLegend Cat. No. 300311) AB_314048 (BioLegend Cat. No. 300312)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
T cells, NKT cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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