Alexa Fluor® 647 anti-human CD3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1344	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
Rivollier A, et al. 2004. Blood 104:4029. (Activ)
Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Knyazhitsky M, et al. 2012. J Biol Chem. 287:19725. PubMed

Product Citations

Knyazhitsky M, et al. 2012. J Biol Chem. 287:19275. PubMed
Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
Harris MJ, et al. 2021. Mol Syst Biol. 17:e10091. PubMed
Bounab Y, et al. 2020. Nature Protocols. 15(9):2920-2955. PubMed
Cho JH, et al. 2021. Nat Commun. 12:792. PubMed

RRID

AB_493692 (BioLegend Cat. No. 300321) AB_493693 (BioLegend Cat. No. 300322)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: T cells, NKT cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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