Alexa Fluor® 700 anti-human CD15 (SSEA-1) Anti-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1488	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections⁵ and formalin-fixed paraffin-embedded tissue sections.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)

Product Citations

Noto PB, et al. 2020. Sci Rep. 10:22155. PubMed
Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
Ambikan AT, et al. 2022. Cell Syst. 13:665. PubMed

RRID

AB_2728238 (BioLegend Cat. No. 301919) AB_2728239 (BioLegend Cat. No. 301920)

Antigen Details

Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: 2526 View all products for this Gene ID
UniProt: View information about CD15 on UniProt.org

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