Spark YG™ 570 anti-human CD45 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark YG? 570 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0?- 10??g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG? 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹,?Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Kishihara K, et al. 1993. Cell 74:143.
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
Nagano M, et al. 2007. Blood 110:151.
Jiang Q, et al. 2008. Blood 112:2858. PubMed
Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
Lee J, et al. 2015. J Exp Med. 212:385. PubMed
Breton G, et al. 2015. J Exp Med. 212:401. PubMed
Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

RRID

AB_2876596 (BioLegend Cat. No. 304070) AB_2876596 (BioLegend Cat. No. 304071)

Antigen Details

Structure

Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)

Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function

TCR and BCR mediated activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Cell Type

Hematopoietic stem and progenitors, Mesenchymal Stem Cells

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

Thomas M. 1989. Annu. Rev. Immunol. 7:339.
Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID

5788 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

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