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首页 > 抗体蛋白 > 抗体
Purified anti-human CD45 Antibody
产品名称：
Purified anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304001
产品应用：
304001
[价格]
规格 价格 库存
25µg ￥ 288 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF?, IHC-P - Verified
WB, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 10 ?g/ml is suggested, if the antibody is not available in a pre-diluted format. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  3. Nagasawa Y, et al. 2021. PLoS One. 16:e0249340. PubMed
  4. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  5. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
  6. de Vargas Roditi L, et al. 2022. Cell Rep Med. 3:100604. PubMed
  7. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  8. Miyamoto DT, et al. 2018. Cancer Discov. 0.533333333. PubMed
  9. Sugimoto H, et al. 2021. AAPS J. 23:36. PubMed
  10. Behrens NE, et al. 2019. MBio. 10:2. PubMed
  11. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  12. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  13. Kuwada K, et al. 2018. J Exp Clin Cancer Res. 37:307. PubMed
  14. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  15. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  16. Bokun V, et al. 2019. PLoS One. 14:e0225767. PubMed
  17. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  18. Zhong M, et al. 2021. J Pers Med. 11:. PubMed
  19. Gao H, et al. 2021. J Virol. :. PubMed
  20. Bhat N, et al. 2015. J Immunol. 195: 5178 - 5188. PubMed
  21. Pir GJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  22. Lu-Culligan A, et al. 2021. Med. 2(5):591-610.e10. PubMed
  23. Chow SH, et al. 2020. J Leukoc Biol. 108:967. PubMed
  24. Aspegren O, et al. 2022. Front Neurol. 13:846293. PubMed
  25. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  26. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  27. Arakelyan A, et al. 2017. Sci Rep. 1.46875. PubMed
  28. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  29. Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
  30. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  31. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  32. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  33. Elst J, et al. 2022. Front Immunol. 13:835618. PubMed
  34. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  35. Nagano M, et al. 2007. Blood . 110:151. PubMed
  36. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  37. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  38. Bottoni G, et al. 2019. Nat Commun. 10:3884. PubMed
  39. B?ttcher C, et al. 2019. Sci Rep. 9:19471. PubMed
  40. Frobel J, et al. 2018. Clin Epigenetics. 10:67. PubMed
  41. Zheng Y, et al. 2022. iScience. 25:103785. PubMed
  42. Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed
  43. Cox MJ, et al. 2021. Mol Ther. 29:1529. PubMed
  44. Cardoso V, et al. 2017. Nature. 549:277. PubMed
  45. Staessens S, et al. 2019. Haematologica. 104(10). PubMed
  46. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  47. Schwabenland M, et al. 2021. Immunity. . PubMed
  48. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  49. Rabilloud T, et al. 2021. Nat Commun. 12:865. PubMed
  50. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  51. Kanayama M, et al. 2021. Oncol Lett. 22:522. PubMed
  52. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  53. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  54. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
  55. Jowett GM, et al. 2021. Nat Mater. 20:250. PubMed
  56. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  57. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  58. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  59. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  60. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  61. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  62. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_314389 (BioLegend Cat. No. 304001) AB_314390 (BioLegend Cat. No. 304002)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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