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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-human CD45 Antibody
产品名称：
PerCP/Cyanine5.5 anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304027
产品应用：
304027
[价格]
规格 价格 库存
25tests ￥ 1908 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  2. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  3. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  4. Zhou L, et al. 2021. MAbs. 13:1964420. PubMed
  5. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
  6. Caggiati A, et al. 2017. Aesthet Surg J. 10.1093/asj/sjw211. PubMed
  7. Gerhauser C et al. 2018. Cancer cell. 34(6):996-1011 . PubMed
  8. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  9. Godoy-Pacheco A, et al. 2022. Oncol Lett. 24:288. PubMed
  10. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  11. Imai Y, et al. 2021. JID Innov. 1:100003. PubMed
  12. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  13. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  14. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  15. Kanderova V, et al. 2016. Mol Cell Proteomics. 15: 1246-1261. PubMed
  16. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  17. Soh KT, et al. 2020. Cytometry B Clin Cytom. 98:161. PubMed
  18. Roug A, et al. 2014. Cytotherapy. 16:392. PubMed
  19. Xu Y, et al. 2021. Cell Reports. 36(6):109524. PubMed
  20. Giraud M, et al. 2012. Proc Natl Acad Sci U S A. 109:535. PubMed
  21. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  22. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  23. Johnson AJ, et al. 2021. Cancer Immunol Res. 9:1047. PubMed
RRID
AB_1236444 (BioLegend Cat. No. 304027) AB_893338 (BioLegend Cat. No. 304028)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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