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PE anti-human CD45 Antibody
产品名称：
PE anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304007
产品应用：
304007
[价格]
规格 价格 库存
25tests ￥ 336 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Vincent V, et al. 2020. Biotechniques. 68:325. PubMed
  2. Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed
  3. Ackermann M, et al. 2018. Nat Commun. 9:5088. PubMed
  4. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  5. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  6. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  7. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  8. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  9. Gumber D, et al. 2020. eLife. 9:00. PubMed
  10. Alisjahbana A, et al. 2021. Front Immunol. 12:752104. PubMed
  11. Witkowski M, et al. 2021. Nature. 600:295. PubMed
  12. Schwabenland M, et al. 2021. Acta Neuropathol. 142:923. PubMed
  13. Tu C, et al. 2021. Theranostics. 2.1. PubMed
  14. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  15. Thompson ER, et al. 2020. Am J Transplant. . PubMed
  16. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  17. Tancharoen W, et al. 2019. Mol Med Rep. 19:5123. PubMed
  18. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  19. Gao G, et al. 2022. Am J Cancer Res. 12:1934. PubMed
  20. Gong Y, et al. 2021. Aging (Albany NY). 13:20629. PubMed
  21. Zhang B, et al. 2022. Stem Cells Int. 2022:2236250. PubMed
  22. Martínez-Sabadell A, et al. 2022. STAR Protoc. 3:101712. PubMed
  23. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  24. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  25. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  26. Weinstein S, et al. 2016. Proc Natl Acad Sci U S A. 113: 16 - 22. PubMed
  27. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  28. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  29. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  30. Morton JJ, et al. 2020. Molecular Cancer Research. 19(2):346-357. PubMed
  31. Singh A, et al. 2020. Mol Oncol. 1.901388889. PubMed
  32. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  33. Binnewies M, et al. 2021. Cell Rep. 37:109844. PubMed
  34. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  35. Yuan J et al. 2018. Molecular cell. 72(2):380-394 . PubMed
  36. Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed
  37. Zhou T, et al. 2022. Sci Adv. 8:eabj9617. PubMed
  38. Cai S, et al. 2022. Cell Biosci. 12:115. PubMed
  39. Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed
  40. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  41. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
  42. Wang W, et al. 2022. Int J Mol Sci. 23:. PubMed
  43. Hu Q, et al. 2021. Nat Commun. 12:2186. PubMed
  44. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  45. Fei D, et al. 2021. Front Cell Dev Biol. 9:687258. PubMed
  46. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  47. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  48. Sato K, et al. 2010. J Virol. 84:9546. PubMed
  49. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  50. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
RRID
AB_314395 (BioLegend Cat. No. 304007) AB_314396 (BioLegend Cat. No. 304008) AB_2564156 (BioLegend Cat. No. 304058) AB_2562057 (BioLegend Cat. No. 304039)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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