Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

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* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹,?Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

1. Knapp W,?et al.?1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K,?et al.?1993.?Cell?74:143.
3. Esser M,?et al.?2001.?J. Virol.?75:6173. (WB)
4. Yamada T,?et al.?2002.?J. Biol. Chem.?277:28830.
5. Nagano M,?et al.?2007.?Blood?110:151.
6. Jiang Q,?et al.?2008.?Blood?112:2858.?PubMed
7. Morozov A,?et al.?2010.?Clin Cancer Res.?16:5630.?PubMed
8. Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
9. Friedman T,?et al.?1999.?J. Immunol.?162:5256. (IHC)
10. Oeztuerk-Winder F,?et al.?2012.?EMBO J.?31:3431. (FC)?PubMed
11. Rees LE,?et al.?2003.?Clin. Exp. Immunol.?134:497. (IF)
12. Lee J,?et al.?2015.?J Exp Med.?212:385.?PubMed
13. Breton G,?et al.?2015.?J Exp Med.?212:401.?PubMed
14. Marquardt N,?et al.?2015.?J Immunol.?6:2467.?PubMed
15. Bushway ME,?et al. 2014.?Biol Reprod. 90(5): 110. (IF)?PubMed
16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

• Batch-to-batch consistency
• Material traceability
• Documented procedures
• Documented employee training
• Equipment maintenance and monitoring records
• Lot-specific certificates of analysis
• Quality audits per ISO 13485:2016
• QA review of released products

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

1. Thomas M. 1989.?Annu. Rev. Immunol.?7:339.
2. Trowbridge I,?et al.?1994.?Annu. Rev. Immunol.12:85.