GMP APC anti-human CD45 Antibody
- 产品名称：
- GMP APC anti-human CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 260034
- 产品应用：
- 260034
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 4164 |
1 |
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 12.5 ?g/mL
- Storage & Handling
- The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, and Western blotting3.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W,?et al.?1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K,?et al.?1993.?Cell?74:143.
- Esser M,?et al.?2001.?J. Virol.?75:6173. (WB)
- Yamada T,?et al.?2002.?J. Biol. Chem.?277:28830.
- Nagano M,?et al.?2007.?Blood?110:151.
- Jiang Q,?et al.?2008.?Blood?112:2858.?PubMed
- Morozov A,?et al.?2010.?Clin Cancer Res.?16:5630.?PubMed
- Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
- Friedman T,?et al.?1999.?J. Immunol.?162:5256. (IHC)
- Oeztuerk-Winder F,?et al.?2012.?EMBO J.?31:3431. (FC)?PubMed
- Rees LE,?et al.?2003.?Clin. Exp. Immunol.?134:497. (IF)
- Lee J,?et al.?2015.?J Exp Med.?212:385.?PubMed
- Breton G,?et al.?2015.?J Exp Med.?212:401.?PubMed
- Marquardt N,?et al.?2015.?J Immunol.?6:2467.?PubMed
- Bushway ME,?et al. 2014.?Biol Reprod. 90(5): 110. (IF)?PubMed
- RRID
- AB_2922505 (BioLegend Cat. No. 260034)
- Disclaimer
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
- Structure
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- Function
- TCR and BCR mediated activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989.?Annu. Rev. Immunol.?7:339.
- Trowbridge I,?et al.?1994.?Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
