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Brilliant Violet 421™ anti-human CD45 Antibo
产品名称：
Brilliant Violet 421™ anti-human CD45 Antibo
产品类别：
抗体
产品编号：
304031
产品应用：
304031
[价格]
规格 价格 库存
25tests ￥ 2280 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  2. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  3. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  4. Murdock BJ, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  5. Maat H, et al. 2021. iScience. 24(5):102435. PubMed
  6. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  7. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  8. Zhang J, et al. 2022. Nature. 609:369. PubMed
  9. Ng E, et al. 2016. Nat Biotechnol. 34:1168-1179. PubMed
  10. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  11. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  12. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  13. Worthen CA, et al. 2020. J Invest Dermatol. 140:2515. PubMed
  14. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  15. Sd W, et al. 2015. Sci Rep. 5: 12270. PubMed
  16. Watanabe J, et al. 2018. Oncotarget. 9:24000. PubMed
  17. Gao X, et al. 2021. J Clin Invest. 131:. PubMed
  18. Ganti SN, et al. 2015. Sci Rep. 5: 12255. PubMed
  19. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  20. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  21. Ruella M, et al. 2018. Nat Med. 24:1499. PubMed
  22. Howden S et al. 2019. Stem Cell Res. 1728:53:00 . PubMed
  23. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  24. Hosseini Far H et al. 2019. Stem Cell Res. 1727:49:00 . PubMed
  25. Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed
  26. Berent-Maoz B, et al. 2015. PLoS One. 10:125414. PubMed
  27. Eckert EC, et al. 2020. Mol Ther Oncolytics. 0.710416667. PubMed
  28. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  29. Pettmann J, et al. 2021. eLife. 10:00. PubMed
  30. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
RRID
AB_10900423 (BioLegend Cat. No. 304031) AB_2561357 (BioLegend Cat. No. 304032)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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