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首页 > 抗体蛋白 > 抗体
APC anti-human CD45 Antibody
产品名称：
APC anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304011
产品应用：
304011
[价格]
规格 价格 库存
25tests ￥ 528 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Park E, et al. 2017. Methods Mol Biol. 10.1007/978-1-4939-7144-2_2. PubMed
  2. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
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  4. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  5. Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed
  6. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  7. Sun YF, et al. 2018. Clin Cancer Res. 24:547. PubMed
  8. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  9. Loreth D, et al. 2021. Int J Mol Sci. 22:. PubMed
  10. Shi L, et al. 2021. Stem Cell Res Ther. 12:147. PubMed
  11. O’Dea K, et al. 2016. PLoS One. 11:e0167801. PubMed
  12. Hultmark S, et al. 2020. Haematologica. . PubMed
  13. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  14. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  15. Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed
  16. Karim F, et al. 2021. Elife. 10:. PubMed
  17. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  18. Cortesi EE, et al. 2021. Cells. 10:. PubMed
  19. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  20. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  21. Vignard V, et al. 2020. Cancer Immunol Res. 0.510416667. PubMed
  22. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
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  25. Gonzalez VD, et al. 2021. Cell Reports. 36(9):109632. PubMed
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  27. Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
  28. Buenafe AC, et al. 2022. Sci Rep. 12:3556. PubMed
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  30. Chen IP, et al. 2020. Bio Protoc. 10:e3854. PubMed
  31. Niu LT, et al. 2021. Transl Oncol. 14:101159. PubMed
  32. Gleason M, et al. 2014. Blood. 123:3016. PubMed
  33. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  34. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  35. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  36. Dong R, et al. 2020. EMBO J. 39:e104730. PubMed
  37. Turetsky A, et al. 2014. Sci Rep. 4:4782. PubMed
  38. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  39. Wi?feld J, et al. 2021. Glia. 69:1393. PubMed
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  41. Nagano M, et al. 2007. Blood . 110:151. PubMed
  42. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  43. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  44. Subedi A, et al. 2021. Cell Stem Cell. :. PubMed
  45. Li CJ, et al. 2018. J Clin Invest. 128:5251. PubMed
  46. Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed
  47. Weitz JR, et al. 2020. Diabetes. 69:1206. PubMed
  48. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  49. de Jonge K, et al. 2021. OncoImmunology. 10(1):1873585. PubMed
  50. Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed
  51. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  52. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  53. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  54. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  55. Racle J et al. 2017. eLife. 6 pii: e26476. PubMed
  56. Nasri M, et al. 2019. Haematologica. 104:. PubMed
  57. He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
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  59. ?yszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  60. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  61. Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed
  62. Delgado-Gonzalez A, et al. 2022. STAR Protoc. 3:101425. PubMed
  63. Luzuriaga J, et al. 2020. Biomedicines. 8:00. PubMed
  64. Kasagi Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:333. PubMed
  65. Morita K, et al. 2020. Nat Commun. 11:5327. PubMed
  66. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  67. Sugimura R, et al. 2020. Biomed Microdevices. 34:22. PubMed
  68. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  69. Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed
  70. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
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  72. Sun YF, et al. 2021. Nat Commun. 12:4091. PubMed
RRID
AB_314399 (BioLegend Cat. No. 304011) AB_314400 (BioLegend Cat. No. 304012) AB_2562049 (BioLegend Cat. No. 304037)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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