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Alexa Fluor® 647 anti-human CD45 Antibody
产品名称：
Alexa Fluor® 647 anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304020
产品应用：
304020
[价格]
规格 价格 库存
25tests ￥ 1344 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
?g sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Patel T, et al. 2022. Aging Cell. 21:e13606. PubMed
  2. Yee–de León JF, et al. 2020. Sci Rep. 5.654861111. PubMed
  3. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  4. Wohl I, et al. 2021. Front Cell Dev Biol. 9:590655. PubMed
  5. Gierahn T, et al. 2017. Nat Methods. 10.1038/nmeth.4179. PubMed
  6. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  7. Jung JY, et al. 2021. Cells. 10:. PubMed
  8. McComb S, et al. 2016. Sci Transl Med. 8: 339ra70. PubMed
  9. Lim H, et al. 2015. J Immunol. 195: 5432 - 5439. PubMed
  10. Razvag Y, et al. 2019. Cell Rep. 29:3506. PubMed
  11. Sajman J, et al. 2021. Commun Biol. 4:439. PubMed
  12. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  13. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  14. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  15. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  16. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  17. Olah M, et al. 2020. Nat Commun. 4.714583333. PubMed
  18. Zhong W, et al. 2016. Nat Commun. 7: 12702. PubMed
  19. Luong-Gardiol N, et al. 2019. Cancer Cell. 35:649. PubMed
  20. Futia G, et al. 2017. Cytometry A. 10.1002/cyto.a.23144. PubMed
  21. Bartkowiak K, et al. 2021. Cancers (Basel). 13:. PubMed
  22. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  23. Chen S, et al. 2017. PLoS One. 10.1371/journal.pone.0181249. PubMed
  24. Raimondi L, et al. 2020. Int J Mol Sci. 21:00. PubMed
  25. Aguilar–Avelar C, et al. 2019. Sci Rep. 9:13766. PubMed
  26. Garcia J, et al. 2019. Cancer Med. 10.1002/cam4.2244. PubMed
  27. Gonzales–Aloy E, et al. 2019. Haematologica. 104:296. PubMed
  28. Perry S, et al. 2011. PLoS One. 7:e1001297. PubMed
  29. Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed
  30. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  31. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  32. Janning M, et al. 2019. Cancers (Basel). 11. PubMed
  33. Olah M, et al. 2018. Nat Commun. 9:539. PubMed
  34. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  35. Beasley AB, et al. 2021. Methods Mol Biol. 2265:213. PubMed
RRID
AB_493034 (BioLegend Cat. No. 304020) AB_2564155 (BioLegend Cat. No. 304056) AB_389336 (BioLegend Cat. No. 304018)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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