Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹,?Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

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15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

1. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

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2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.