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Alexa Fluor® 488 anti-human CD45 Antibody
产品名称：
Alexa Fluor® 488 anti-human CD45 Antibody
产品类别：
抗体
产品编号：
304019
产品应用：
304019
[价格]
规格 价格 库存
25tests ￥ 1296 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11,?Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. ?zcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  2. Earl DC, et al. 2018. Nat Commun. 9:39. PubMed
  3. Johnson KC, et al. 2021. Nat Genet. 53:1456. PubMed
  4. O'Donovan B, et al. 2020. Brain Commun. 2:fcaa059. PubMed
  5. Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
  6. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  7. VanderBurgh JA, et al. 2018. Integr Biol (Camb). 0.926388889. PubMed
  8. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  9. Meehan S, et al. 2019. Commun Biol. 2:229. PubMed
  10. Gonzales L, et al. 2015. J Histochem Cytochem. 63: 908 - 921. PubMed
  11. Mellergaard M, et al. 2020. J Biol Chem. 295:11803. PubMed
  12. Man L, et al. 2020. Cell Reports. 32(6):108027. PubMed
  13. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  14. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  15. Brian BF, et al. 2022. Sci Rep. 12:5875. PubMed
  16. Hendricks A, et al. 2020. Cancers (Basel). 12:00. PubMed
  17. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  18. Ji S, et al. 2021. Clin Sci (Lond). 135:1127. PubMed
  19. Liu T, et al. 2022. J Hematol Oncol. 15:141. PubMed
  20. Li TJ, et al. 2021. Int J Cancer. 148:1756. PubMed
  21. Bashour K, et al. 2014. Proc Natl Acad Sci U S A. 111:2241. PubMed
  22. Takeda A, et al. 2020. STAR Protoc. 1:100021. PubMed
  23. Farshidfar F, et al. 2022. Nat Commun. 13:898. PubMed
RRID
AB_493033 (BioLegend Cat. No. 304019) AB_389314 (BioLegend Cat. No. 304017)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

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