Biotin anti-human CD11a Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2256	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cow, Capuchin monkey, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin⁸. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting², and blocking of cell-cell interaction and inhibition the binding of ICAM-1⁴. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301233 & 301234).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
Leite F, et al. 2002. Infec. Immun. 70:4336.
Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
BTchard D, et al. 2001. J. Immunol. 167:3099.
Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
Choi EY, et al. 2008. Blood 111:3607. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Ma Q, et al. 2002. J. Biol. Chem. 277:10638.

RRID

AB_314142 (BioLegend Cat. No. 301204)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β₂ (CD18) forms LFA-1, 170-180 kD
Distribution: Leukocytes
Function: Adhesion, costimulation
Ligand/Receptor: ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
Gene ID: 3683 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们