APC anti-human CD11a Antibody
- 产品名称：
- APC anti-human CD11a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301212
- 产品应用：
- 301212
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 3276 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cow, Capuchin monkey, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301233 & 301234).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Leite F, et al. 2002. Infec. Immun. 70:4336.
- Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
- BTchard D, et al. 2001. J. Immunol. 167:3099.
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Choi EY, et al. 2008. Blood 111:3607. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Ma Q, et al. 2002. J. Biol. Chem. 277:10638.
- Product Citations
-
- Davidson T, et al. 2022. Adv Radiat Oncol. 7:100931. PubMed
- Zhang H, et al. 2021. Clin Cancer Res. 27:6376. PubMed
- Vera Aguilera J, et al. 2020. Melanoma Res. 30:364. PubMed
- Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
- Yan Y, et al. 2018. JCI Insight. 3. PubMed
- RRID
- AB_314150 (BioLegend Cat. No. 301212)
- Structure
- Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
- Distribution
-
Leukocytes
- Function
- Adhesion, costimulation
- Ligand/Receptor
- ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
- Gene ID
- 3683 View all products for this Gene ID
- UniProt
- View information about CD11a on UniProt.org
