PerCP/Cyanine5.5 anti-human CD45RA Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304121
- 产品应用：
- 304121
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1692 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The CD45RA antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Sammicheli S, et al. 2012. J Autoimmun. 38:304. PubMed
- Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
- Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
- Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
- Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
- Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
- Li M, et al. 2022. Sci Rep. 12:378. PubMed
- Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
- Selleri S, et al. 2011. J Allergy Clin Immunol. 127:1368. PubMed
- de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
- Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
- Liu Y, et al. 2018. JCI Insight. 3:. PubMed
- Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
- Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed
- Iannello A, et al. 2010. J Immunol. 184:114. PubMed
- Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
- Brandsma AM, et al. 2021. Cancer Discov. . PubMed
- Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
- Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
- Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
- Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
- Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed
- Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed
- Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
- Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
- Ogonek J, et al. 2017. J Immunol. 10.4049/jimmunol.1601992. PubMed
- Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
- Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
- Georg P, et al. 2022. Cell. 185:493. PubMed
- Bunse C, et al. 2013. PLoS One. 8:77925. PubMed
- Bacher P, et al. 2013. J Immunol. 190:3967. PubMed
- Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
- Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
- Talvard-Balland N, et al. 2021. J Invest Dermatol. 141:193. PubMed
- Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
- Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
- Gray DH, et al. 2022. Mol Ther Nucleic Acids. 27:505. PubMed
- Aalam F, et al. 2020. PLoS Pathog. 16:e1008968. PubMed
- RRID
- AB_893358 (BioLegend Cat. No. 304121) AB_893357 (BioLegend Cat. No. 304122)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
