PE anti-human CD45RA Antibody
- 产品名称：
- PE anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304107
- 产品应用：
- 304107
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed
- Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
- Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
- Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
- Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
- Nagle VL, et al. 2022. Mol Cancer Ther. 21:658. PubMed
- de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
- Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
- Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
- Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
- Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
- Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
- Zhang M, et al. 2020. J Cell Mol Med. . PubMed
- Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
- Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed
- Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
- Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
- Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
- Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
- Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
- Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
- Hale M, et al. 2017. Mol Ther Methods Clin Dev. 0.3. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
- Hwang HJ, et al. 2021. Immun Inflamm Dis. 9:274. PubMed
- Kim ML, et al. 2021. iScience. 24:103509. PubMed
- RRID
- AB_314411 (BioLegend Cat. No. 304107) AB_314412 (BioLegend Cat. No. 304108)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
