Pacific Blue™ anti-human CD45RA Antibody
- 产品名称：
- Pacific Blue™ anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304117
- 产品应用：
- 304117
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- test size: lot-specific; ?g sizes: 0.5 mg/ml
- Storage & Handling
- The CD45RA antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 20 ?l per million cells in 100 ?l volume.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 ?g per million cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Han L, et al. 2012. PLoS One. 4:e7989. PubMed
- He X, et al. 2017. Sci Rep. 7:43003. PubMed
- Rajamanickam A, et al. 2018. PLoS Negl Trop Dis. 12:e0006481. PubMed
- Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
- Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
- Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed
- Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
- Travaglini KJ, et al. 2020. Nature. 587:619. PubMed
- Blatt K, et al. 2014. Clin Cancer Res. 20:3589. PubMed
- Lo Presti V, et al. 2021. Mol Ther Methods Clin Dev. 21:357. PubMed
- Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
- Chen H, et al. 2021. iScience. 24:103387. PubMed
- Derhovanessian E, et al. 2010. J Immunol. 185:4618. PubMed
- Liisborg C, et al. 2021. Aging (Albany NY). 13:25763. PubMed
- Kurktschiev P, et al. 2014. J Exp Med. 211:2047. PubMed
- Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed
- Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
- Trinité B, et al. 2014. PLoS One. 9:110719. PubMed
- Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
- Pellicanò M, et al. 2014. J Gerontol A Biol Sci Med Sci. 69:495. PubMed
- RRID
- AB_493656 (BioLegend Cat. No. 304117) AB_2174122 (BioLegend Cat. No. 304123) AB_493657 (BioLegend Cat. No. 304118)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
