GMP FITC anti-human CD45RA Antibody
- 产品名称：
- GMP FITC anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 260044
- 产品应用：
- 260044
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 2820 |
1 |
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 200 ?g/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3?and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W,?et al.?1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T,?et al.?2002.?J. Biol. Chem.?277:28830. (WB, Block)
- Weninger W,?et al.?2003?J. Immunol.?170:4638. (IHC-F)
- Imanguli MM,?et al.?2009.?Blood. 113:3620 (IHC-P)
- Roque S,?et al.?2007. J. Immunol.?178:8028. (FC)?PubMed
- Smeltz RB. 2007.?J. Immunol.?178:4786. (FC)?PubMed
- Palendira U,?et al.?2008.?Blood?(FC)?PubMed
- Kuttruff S,?et al.?2009.?Blood?113:358. (FC)?PubMed
- Thakral D,?et al.?2008.?J. Immunol.?180:7431. (FC)?PubMed
- Alanio C,?et al.?2010.?Blood?115:3718. (FC)?PubMed
- Iannello A,?et al.?2010.?J. Immunol.?184:114. (FC)?PubMed
- Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
- Guereau-de-Arellan M,?et al.?2011.?Brain. 134:3578.?PubMed
- Canque B,?et al.?2000.?Blood?96:3748. (ICC)
- Imanguli MM,?et al.?2009.?Blood?13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
- AB_2910359 (BioLegend Cat. No. 260044)
- Disclaimer
GMP RUO Flow Cytometry Antibodies.?BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
