FITC anti-human CD45RA Antibody
- 产品名称：
- FITC anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304105
- 产品应用：
- 304105
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- ?g sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
- Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
- Ge Y, et al. 2016. Diabetes. 65: 794 - 802. PubMed
- Bachmann M, et al. 2012. J Biol Chem. 287:41684. PubMed
- Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
- Sun J, et al. 2018. Scand J Immunol. 88:e12674. PubMed
- Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
- Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
- Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
- Weithauser A, et al. 2013. J Am Coll Cardiol. 62:1737. PubMed
- Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
- Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed
- Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
- Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
- Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
- Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
- Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed
- Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
- Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
- Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed
- Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
- Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
- Li Q, et al. 2012. J Leukoc Biol. 91:311. PubMed
- Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
- Su X, et al. 2022. J Transl Med. 20:378. PubMed
- Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
- Cesana M, et al. 2018. Cell Stem Cell. 22:575. PubMed
- Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
- Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
- Yuan J et al. 2018. Molecular cell. 72(2):380-394 . PubMed
- Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed
- Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
- Lancioni C, et al. 2011. Infect Immun. 79:663. PubMed
- Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
- Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
- Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
- Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed
- Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
- Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
- Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
- Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
- Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
- Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
- Li M, et al. 2020. Nat Commun. 4051:11. PubMed
- RRID
- AB_314409 (BioLegend Cat. No. 304105) AB_314410 (BioLegend Cat. No. 304106) AB_2564157 (BioLegend Cat. No. 304148)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
