Brilliant Violet 421™ anti-human CD45RA Anti
- 产品名称：
- Brilliant Violet 421™ anti-human CD45RA Anti
- 产品类别：
- 抗体
- 产品编号：
- 304129
- 产品应用：
- 304129
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
- Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
- Mo Y, et al. 2022. Front Immunol. 12:799896. PubMed
- Graav G, et al. 2016. PLoS One. 11: 0148604. PubMed
- Balakrishnan A, et al. 2017. Biol Blood Marrow Transplant. 10.1016/j.bbmt.2017.07.016. PubMed
- Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
- Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
- Claireaux M, et al. 2018. MBio. 9:e00317. PubMed
- Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed
- Sckisel G, et al. 2017. J Immunother Cancer . 10.1186/s40425-017-0235-4. PubMed
- Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
- Lichtenegger FS, et al. 2018. Front Immunol. 9:385. PubMed
- Jo T, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
- Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
- Thommen D, et al. 2015. Cancer Immunol Res. 3: 1344 - 1355. PubMed
- Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
- Mukhopadhyay M, et al. 2017. J Immunol. 10.4049/jimmunol.1700953. PubMed
- Xu S, et al. 2021. Immunity. . PubMed
- Yankova E, et al. 2021. Nature. 593:597. PubMed
- Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
- Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
- Mudd PA, et al. 2022. Cell. 185:603. PubMed
- Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed
- Sekine T, et al. 2020. Cell. 183:158. PubMed
- Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
- Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
- Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed
- Schouest B, et al. 2021. J Virol. 95:. PubMed
- Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
- Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
- Ryan J, et al. 2016. Proc Natl Acad Sci U S A. 113: 1286 - 1295. PubMed
- Yu ED, et al. 2022. Cell Host Microbe. . PubMed
- Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
- RRID
- AB_10900421 (BioLegend Cat. No. 304129) AB_10965547 (BioLegend Cat. No. 304130)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
