Alexa Fluor® 700 anti-human CD45RA Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD45RA Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304119
- 产品应用：
- 304119
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
- Payne R, et al. 2010. J Virol. 84:10453. PubMed
- Afzali B, et al. 2013. Clin J Am Soc Nephrol. 8:1396. PubMed
- Karim F, et al. 2021. Elife. 10:. PubMed
- Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
- Eberhardt K, et al. 2015. Clin Infect Dis. 61: 1615 - 1623. PubMed
- Eriksson E, et al. 2012. PLoS One. 7:e51696. PubMed
- Carney E, et al. 2012. J Immunol. 189:261. PubMed
- Alanio C, et al. 2010. Blood. 115:3718. PubMed
- Muenchhoff M, et al. 2016. Sci Transl Med. 8: 358ra125. PubMed
- Alexander T, et al. 2013. Ann Rheum Dis. 72:1549. PubMed
- Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
- Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
- Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed
- Suwandi JS, et al. 2020. J Autoimmun. 107:102361. PubMed
- Powell R, et al. 2017. J Immunol. 10.4049/jimmunol.1700114. PubMed
- Leclercq G, et al. 2021. J Immunother Cancer. 9: . PubMed
- Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
- Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed
- Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
- Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
- Leclercq G, et al. 2022. J Immunother Cancer. 10:. PubMed
- Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
- Palendira U, et al. 2008. Blood. 112:3293. PubMed
- Kim Y, et al. 2021. Oncol Lett. 1.022222222. PubMed
- Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
- Goc J, et al. 2021. Cell. :. PubMed
- Long H, et al. 2013. J Exp Med. 210:933. PubMed
- RRID
- AB_493762 (BioLegend Cat. No. 304119) AB_493763 (BioLegend Cat. No. 304120)
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, na?ve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
