Alexa Fluor® 647 anti-human/mouse Cutaneous Ly-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1212	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes⁴. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin³, and immunohistochemistry^1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References (PubMed link indicates BioLegend citation)

Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
Picker LJ, et al. 1991. Nature 349:796. (IHC)
Berg EL, et al. 1991. J. Exp. Med. 174:1461.
Borges E, et al. 1997. J. Biol. Chem. 272:28786.
Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed

Product Citations

Kuri-Cervantes L, et al. 2020. STAR Protoc. 1:100154. PubMed
Colomb F, et al. 2020. Cell Reports. 32(5):107991. PubMed

RRID

AB_2563661 (BioLegend Cat. No. 321309) AB_2563662 (BioLegend Cat. No. 321310)

Antigen Details

Structure: 140 kD, carbohydrate epitope of PSGL-1
Distribution: T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes
Function: Memory cells tethering and rolling
Ligand/Receptor: E-selectin, P-selectin, L-selectin
Cell Type: B cells, Dendritic cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.
Gene ID: 6404 View all products for this Gene ID
UniProt: View information about CLA on UniProt.org

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