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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 594 anti-human/mouse Cutaneous Ly
产品名称：
Alexa Fluor® 594 anti-human/mouse Cutaneous Ly
产品类别：
抗体
产品编号：
321317
产品应用：
321317
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
  2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
  3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
  4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
  5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed
Product Citations
  1. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
RRID
AB_2734303 (BioLegend Cat. No. 321317)

Antigen Details

Structure
140 kD, carbohydrate epitope of PSGL-1
Distribution

T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes

Function
Memory cells tethering and rolling
Ligand/Receptor
E-selectin, P-selectin, L-selectin
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.

Gene ID
6404 View all products for this Gene ID
UniProt
View information about CLA on UniProt.org

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