TotalSeq™-B0814 anti-human CD205 (DEC-205) A-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised against a purified extracellular domain of human CD205-IgG1 Fc fusion protein.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-B oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of fixed, frozen sections⁴ and Western blotting⁴.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Guo M, et al. 2000. Human Immunology 61:729.
Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
Gurer C, et al. 2008. Blood 112:1231. PubMed
Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)

RRID

AB_2876648 (BioLegend Cat. No. 342215)

Antigen Details

Structure: Macrophage mannose receptor family, C-type lectin, 210 kD
Distribution: High levels on dendritic cells and thymic epithelial cells, low levels on lymphocytes and monocytes
Function: Antigen uptake and process
Cell Type: Dendritic cells, Epithelial cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Guo M, et al. 2000. Human Immunology 61:729.
2. Kato M, et al. 2006. Int. Immunol.
3. Kato M, et al. 2003. J. Biol. Chem. 278(36):34035.
4. Small M and G. Kraal. 2003. Int. Immunol. 15(2):197.
5. Mahnke K, et al. 2000. J. Cell Biol. 151(3):673.
Gene ID: 4065 View all products for this Gene ID
UniProt: View information about CD205 on UniProt.org

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