Alexa Fluor® 488 anti-human CD54 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-human CD54 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 322713
- 产品应用：
- 322713
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1488 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 1 million cells or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of lymphoctes interaction1. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 322721 & 322722).
- Application References
(PubMed link indicates BioLegend citation) -
- Evans HG, et al. 2009. Proc. Natl. Acad. Sci. USA. 106:6232. (Block) PubMed
- Product Citations
-
- Morad G, et al. 2020. International Journal of Molecular Sciences. 21(11):3851. PubMed
- Silva-Filho JL, et al. 2021. Elife. 10:. PubMed
- Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
- Bailey KA, et al. 2019. FASEB J. 33:12888. PubMed
- Rajamani A, et al. 2019. Sci Rep. 6.385416667. PubMed
- Toda H, et al. 2020. Nat Commun. 2.375694444. PubMed
- King D, et al. 2019. Sci Rep. 9:4157. PubMed
- Kleine M, et al. 2014. PLoS One. 9:99713. PubMed
- Ambrose AR, et al. 2020. Proc Natl Acad Sci U S A. 117:23717. PubMed
- Sei Y, et al. 2017. Sci Rep. 10.1038/s41598-017-10636-z. PubMed
- RRID
- AB_535985 (BioLegend Cat. No. 322713) AB_535986 (BioLegend Cat. No. 322714)
- Structure
- Type I membrane protein, Ig superfamily, 85-110 kD
- Distribution
-
Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.
- Function
- Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
- Ligand/Receptor
- CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Voraberger G, et al.. 1991 J. Immunol. 147:2777.
2. Staunton DE, et al.. 1988. Cell 52:925.
3. Greve JM, et al.. 1989. Cell 56:839.
- Gene ID
- 3383 View all products for this Gene ID
- UniProt
- View information about CD54 on UniProt.org
