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PE/Cyanine7 anti-human CD14 Antibody
产品名称:
PE/Cyanine7 anti-human CD14 Antibody
产品类别:
抗体
产品编号:
325617
产品应用:
325617
[价格]
规格 价格 库存
25tests ¥ 1836 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Wang H, et al. 2020. Cell Stem Cell. 28(3):535-549.e8. PubMed
  2. Miggitsch C, et al. 2019. EBioMedicine. 46:387. PubMed
  3. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  4. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  5. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  6. Lucas C, et al. 2020. Nature. 584:463. PubMed
  7. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  8. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  9. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  10. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  11. Vetsika EK, et al. 2021. Cancers (Basel). 13:. PubMed
  12. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  13. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  14. Gillissen MA, et al. 2018. Blood. 131:131. PubMed
  15. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  16. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  17. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  18. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  19. Koga H, et al. 2018. Front Immunol. 1.456944444. PubMed
  20. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  21. Haller PM, et al. 2020. Biomedicines. 8:. PubMed
  22. Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed
  23. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  24. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  25. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  26. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  27. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  28. Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
  29. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
RRID
AB_830690 (BioLegend Cat. No. 325617) AB_830691 (BioLegend Cat. No. 325618)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

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