Pacific Blue™ anti-human CD14 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 325615
- 产品应用：
- 325615
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?The suggested use of this reagent is ≤ 2.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Product Citations
-
- Johnson L, Olson B, and McNeel D. 2017. J Immunother Cancer. 10.1186/s40425-017-0260-3. PubMed
- Johnson LE, et al. 2017. J Immunother Cancer. 5:56. PubMed
- Davis Z, et al. 2011. J Vis Exp. 49: 2668. PubMed
- Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
- Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
- Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
- Crespo ?C, et al. 2020. Cell. 182(5):1125-1139. PubMed
- Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
- Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
- Demirel &, et al. 2007. J Biol Chem. 282:37836. PubMed
- Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
- Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed
- Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
- Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
- Li G, et al. 2019. J Virol. 93:e00233-19. PubMed
- Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
- Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
- Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
- Roerden M, et al. 2021. Cancers. 13(11):. PubMed
- Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
- Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
- Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
- RRID
- AB_830688 (BioLegend Cat. No. 325615) AB_830689 (BioLegend Cat. No. 325616)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
