Biotin anti-human CD14 Antibody
- 产品名称：
- Biotin anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 325623
- 产品应用：
- 325623
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
Cell Separation (MojoSort?) - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. For magnetic cell separation with?MojoSort? streptavidin Nanobeads (Cat. No. 480015/480016), the suggested use of this reagent is?≤20 ?g per 10 million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Product Citations
-
- Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
- Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
- Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
- Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
- Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- RRID
- AB_2074053 (BioLegend Cat. No. 325623) AB_2074052 (BioLegend Cat. No. 325624)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
