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Alexa Fluor® 488 anti-human CD14 Antibody
产品名称:
Alexa Fluor® 488 anti-human CD14 Antibody
产品类别:
抗体
产品编号:
325610
产品应用:
325610
[价格]
规格 价格 库存
100tests ¥ 3180 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  2. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  3. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  4. Carlucci PM, et al. 2018. JCI Insight. 3. PubMed
  5. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  6. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  7. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  8. Talbot H, et al. 2020. Sci Rep. 10:12572. PubMed
  9. Fredriksson K, et al. 2014. J Immunol. 192:4497. PubMed
  10. Breedveld AC, et al. 2021. Front Immunol. 12:651049. PubMed
  11. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  12. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
RRID
AB_830683 (BioLegend Cat. No. 325610)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

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