Purified anti-human C3AR Antibody
- 产品名称:
- Purified anti-human C3AR Antibody
- 产品类别:
- 抗体
- 产品编号:
- 345802
- 产品应用:
- 345802
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- hC3aR transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2.
- Application References
(PubMed link indicates BioLegend citation) -
- Soruri A, et al. 2002. J. Immunol. 170:3306 (FC)
- Kiafard Z, et al. 2007 Immunobiology. 212:129 (FC, IHC)
- Product Citations
-
- Bonetti P, et al. 2013. Blood. 122:2233. PubMed
- RRID
- AB_2066747 (BioLegend Cat. No. 345802)
- Structure
- 482 aa, single chain protein with seven membrane-spanning regions, 54 kD
- Distribution
-
Neutrophils, eosinophils, mast cells, monocytes, dendritic cells, hematopoietic and mesenchymal stem cells, endothelial cells
- Function
- After C3a binding, the C3AR signaling results in chemotaxis, granule enzyme release and superoxide anion production.
- Interaction
- G proteins
- Bioactivity
- Chemotaxis, degranulation
- Cell Type
- Dendritic cells, Endothelial cells, Eosinophils, Mast cells, Mesenchymal Stem Cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
1. Wysoczynski M et al. 2009. Leukemia. 23:1455
2. Schraufstatter IU. et al. 2009. J. Immunol. 182:3827
3. Honczarenko M et al. 2005. J. Immunol. 175:3698
4. Reca R et al. 2003. Blood 101:3784
- Gene ID
- 719 View all products for this Gene ID
- UniProt
- View information about C3aR on UniProt.org