Ultra-LEAF™ Purified anti-human CD40 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1392	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis.¹ Alone, or in combination with TLR ligands, clone HIB14 stimulates B cells to produce IL-10 and differentiates it into regulatory B10 (IL-10 producing B cells).⁷ The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 313019 & 313020).

Application References (PubMed link indicates BioLegend citation)

Pound JD, et al. 1999. Int. Immunol. 11:11. (Costim)
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Armengol MP, et al. 2001. Am. J. Pathol. 159:861.
Cavanagh LL, et al. 2005. Arthritis Res. Ther. 7:R230.
Jayakumar A, et al. 2008. Infect Immun.76:2138. PubMed
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Iwata Y, et al. 2011. Blood. 117:530. PubMed

RRID

AB_2814163 (BioLegend Cat. No. 313019) AB_2814164 (BioLegend Cat. No. 313020)

Antigen Details

Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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