Purified anti-human CD54 Antibody
- 产品名称：
- Purified anti-human CD54 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 353101
- 产品应用：
- 353101
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Colonic cancer BM314 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC?- Verified
IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD543. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
- Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
- Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Behrens NE, et al. 2019. MBio. 10:2. PubMed
- Sawada J, et al. 2021. Am J Pathol. 191:396. PubMed
- Poventud-Fuentes I, et al. 2021. Small. 17:e2004889. PubMed
- Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Vazquez Rodriguez G, et al. 2017. Cancer Immunol Res. 10.1158/2326-6066.CIR-16-0150. PubMed
- Kim N, et al. 2020. Cancers (Basel). 12:. PubMed
- Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
- Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed
- Nishihara H, et al. 2021. STAR Protocols. 2(2):100563. PubMed
- RRID
- AB_11204422 (BioLegend Cat. No. 353101) AB_11204426 (BioLegend Cat. No. 353102)
- Structure
- Type I membrane protein, Ig superfamily, 85-110 kD
- Distribution
-
Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.
- Gene ID
- 3383 View all products for this Gene ID
- UniProt
- View information about CD54 on UniProt.org
