Pacific Blue™ anti-human CD54 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD54 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 353109
- 产品应用：
- 353109
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1764 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Colonic cancer BM314 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD543. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
- Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
- Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
- Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
- Vanoni G, et al. 2021. eLife. 10:00. PubMed
- RRID
- AB_10900822 (BioLegend Cat. No. 353109) AB_10900234 (BioLegend Cat. No. 353110)
- Structure
- Type I membrane protein, Ig superfamily, 85-110 kD
- Distribution
-
Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.
- Gene ID
- 3383 View all products for this Gene ID
- UniProt
- View information about CD54 on UniProt.org
