Alexa Fluor® 647 anti-human CD54 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1416	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Colonic cancer BM314 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested
SB -?Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD54³. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)^4,5.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

RRID

AB_2715941 (BioLegend Cat. No. 353113) AB_2715942 (BioLegend Cat. No. 353114)

Antigen Details

Structure: Type I membrane protein, Ig superfamily, 85-110 kD
Distribution: Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.
Gene ID: 3383 View all products for this Gene ID
UniProt: View information about CD54 on UniProt.org

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