Ultra-LEAF™ Purified anti-human CD63 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD63 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 353039
- 产品应用：
- 353039
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- T cell line HPB-ALL
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-P - Verified
ICC, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.
- Application References
(PubMed link indicates BioLegend citation) -
- Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
- Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
- Product Citations
-
- Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
- Porter RL, et al. 2022. J Clin Invest. :. PubMed
- Zabegina L, et al. 2021. Cells. 10:. PubMed
- Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- Lang JB, et al. 2022. Front Oncol. 12:949261. PubMed
- RRID
- AB_2800940 (BioLegend Cat. No. 353039) AB_2800941 (BioLegend Cat. No. 353040)
- Structure
- Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
- Distribution
-
Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle
- Function
- Platelet activation
- Interaction
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Azorsa DO, et al. 1991. Blood 78:280.
- Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
- Hildreth JE, et al. 1991. Blood 77:121.
- Anzai N, et al. 2002. Blood 99:4413.
- Gene ID
- 967 View all products for this Gene ID
- UniProt
- View information about CD63 on UniProt.org
