Ultra-LEAF™ Purified anti-mouse TCR β c-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
IHC-F, IP, Costim, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References (PubMed link indicates BioLegend citation)

Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed

RRID

AB_2813970 (BioLegend Cat. No. 109253) AB_2813971 (BioLegend Cat. No. 109254) AB_2813972 (BioLegend Cat. No. 109255) AB_2813973 (BioLegend Cat. No. 109256) AB_2813974 (BioLegend Cat. No. 109257) AB_2813975 (BioLegend Cat. No. 109258)

Antigen Details

Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound-MHC class I and II
Antigen References: 1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.
Gene ID: 21577 View all products for this Gene ID
UniProt: View information about TCR beta chain on UniProt.org

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